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Internship file from the yr 2014 within the topic Chemistry - Bio-chemistry, grade: 1,0, loose college of Berlin (Institut für Chemie und Biochemie), path: Methodenmodul Nukleinsäuren, language: English, summary: Aptamers may be regarded as nucleic acid established analogues of monoclonar antibodies. those DNA or RNA molecules are characterised by way of a excessive affinity and selectivity to the objective in addition to a big number of attainable ambitions, yet function major merits in comparison to antibodies akin to the shortcoming of immunogenicity and monetary in vitro generation.
The guidance of aptamers relies on systematic evolution (see determine 1). Cycles of choice and replication are performed to slim down an important library to aptamers with the simplest binding houses to the target.
In this test, an RNA aptamer to the rPrPc protein is chosen in vitro. on account that a complete SELEX techniques takes numerous weeks, just one choice cycle is finished examplarily.

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